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abscript ii cdna first-strand synthesis kit  (ABclonal Biotechnology)

 
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    ABclonal Biotechnology abscript ii cdna first-strand synthesis kit
    a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of <t>cDNA</t> library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.
    Abscript Ii Cdna First Strand Synthesis Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abscript ii cdna first-strand synthesis kit/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    abscript ii cdna first-strand synthesis kit - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolution"

    Article Title: CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolution

    Journal: Nature Communications

    doi: 10.1038/s41467-025-61697-y

    a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of cDNA library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.
    Figure Legend Snippet: a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of cDNA library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.

    Techniques Used: cDNA Library Assay, Sequencing



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    abscript ii cdna first-strand synthesis kit rk20400  (ABclonal Biotechnology)
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    a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of <t>cDNA</t> library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of cDNA library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.

    Journal: Nature Communications

    Article Title: CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolution

    doi: 10.1038/s41467-025-61697-y

    Figure Lengend Snippet: a CompasSeq implements a parallel experimental pipeline to simultaneously capture NCIN-capped RNAs and all-capped RNAs (NCIN- and m 7 G-capped RNAs). Meanwhile, CompasSeq implements a dual spike-in strategy to quantify NCIN-capped RNAs. A set of synthetic NCIN-RNAs with known capping ratios, is introduced at the beginning to serve as internal controls. Adapter-pre-ligated Drosophila RNA, is added at the step of cDNA library construction for subsequent computational analysis. b Diagram illustrating the workflow of CompassAnalyzer. CompassAnalyzer first applies stepwise normalizations to recover the intrinsic RNA abundance distribution between NCIN-capped and all-capped RNAs from sequencing data. It then employs empirical Bayes shrinkage to enable quantification of NCIN capping ratios at the transcript level.

    Article Snippet: Reverse transcription was performed using designed primers listed in Supplementary Data with the ABScript II cDNA First-Strand Synthesis Kit (ABclonal, catalog: RK20400).

    Techniques: cDNA Library Assay, Sequencing